Luckily for you my avid followers, you get an extra blog written by me. I slept in and missed a phage hunt lab last semester and in order for it not to affect my grades this is what I had to do. On another note I am very excited to announce that Bazzle was one of three phages in the 2019 phage hunt crew to be sequenced.
The results from the restriction digest gel electrophoresis gave me an idea on how Bazzle differed from the other phages in the phage hunt, however the results from the sequencing indefinitely confirms how much different Bazzle is. All the sequencing information for Bazzle can be found on the phageDB1. For starters the genome length was a whooping 76,734 bp long which is an estimated 2,000 more than the two other sequenced phages, strongarm and squee. Bazzle is also found to be in cluster L with 53 other discovered phages around the world and subcluster L2 shared with 23 other phages. According to phageDB, a phage in the L cluster is typically temperate. Temperate phages are phages that produce lysogens and can choose between the lytic and lysogenic pathways2.
Bazzle had a GC content of 58.7% and an overhang length of 10 bases containing the sequence TCGATCAGCC. Research suggests 3 that there is a correlation between the genome length and the GC content, the longer the genome length, the higher the GC content. Because my genome length is quite long this could be the reason why my GC content is 58.7%. However when comparing my results to other phages this reasoning is quickly thrown out the window. Phages with higher GC content tended to have shorter genome lengths. As you can see science is still learning about the size, shape and uses for phages. I am very excited to be part of this new frontier and delighted that Bazzle is unique.
From the results retrieved from the sequencing, I can’t decipher a lot of what it means in terms of my phage information. This is why I am very excited for this semester where annotation will take place. I cannot wait to see what all this information means and what new information I will discover about Bazzle.
Stay tuned for my next blog which will probably be about what I find during annotation.
2. Phage consultants. (n.d.). Temperate phages.Retrieved from http://www.phageconsultants.com/temperate-phages,16,pl.html
3. Almpanis, A., Swain, M., Gatherer, D., & McEwan, N. (2018). Correlation between bacterial G+ C content, genome size and the G+ C content of associated plasmids and bacteriophages. Microbial genomics, 4(4).